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Volume 13 Journal of Orthopaedics Trauma Surgery & Related Research Arthroplasty 2018
September 24-25, 2018
ArthroplastySeptember 24-25, 2018 London, UK
11th International Conference on
Bioprospecting of marine resources for biopolymer production
Sourish Bhattacharya
CSIR-Central Salt and Marine Chemicals Research Institute, India
West coast of India can be considered as important area for collecting diverse marine microorganisms with biopolymer producing
capacity. In this attempt, 200 marine isolates from west coast of India was obtained, out of which one strain CSMCRI’s Bacillus
licheniformis PL26 was found to be potential for producing ε-polylysine and polyhydroxyalkanoate simultaneously in same
fermentation medium. The PHA produced by Bacillus licheniformis was found to be Poly-3-Hydroxybutyrate (P3HB). Further, in
order to improve the ε-polylysine production, the carbon source was replaced with glucose which yielded 1.2 g L-1 ε-polylysine as
oxygen transfer rate is very low in the medium containing crude glycerol. Further, an advanced modeling and optimization technique
was applied to optimize medium parameters for enhanced ε-polylysine production by marine bacterium Bacillus licheniformis.
The critical nutrients including glucose, yeast extract, magnesium sulfate and ferrous sulfate were incorporated in Artificial Neural
Networks (ANN) as input variables and ε-polylysine as the output variable. The ANN topology of 4-10-1 was found to be optimum
upon training the model with feed-forward back propagation algorithm and on application of the developed model to particle swarm
optimization resulted in 3.56±0.16 g L-1 of ε-polylysine under the following optimal conditions: Glucose 34 g L-1; Yeast extract 2.3
g L-1; Magnesium sulfate 0.44 g L-1 and Ferrous sulfate 0.08 g L-1. Thus, this optimization technique could significantly improve
ε-polylysine by 196.7% as compared to un-optimized medium.
Biography
Sourish Bhattacharya is a Junior Scientist at Process Design and Engineering Cell Discipline in CSIR-Central Salt and Marine Chemicals Research Institute, India.
Sourish Bhattacharya, J Arthroplasty 2018, Volume 13